O-13: Gene Expression Pattern of Adiponectin and Adiponectin Receptors in Bovine Dominant and Non-Dominant Follicles
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Abstract:
Background: Recent studies have revealed that adiponectin, a novel adipocytokine, and its two receptors (AdipoR1 and AdipoR2) are involved in regulation of ovarian function in various farm animal species. The aim of this study was to investigate the gene expression pattern of Adiponectin and its receptors in ovarian cells of dominant (DFs) and non- dominant follicles (NDFs) in the bovine. Materials and Methods: Based on the estradiol/progesterone ratio, two largest follicles from ovaries collected at a slaughterhouse were classified as NDFs and DFs. In addition, the stage of the estrous cycle (follicular or luteal phases) of the slaughtered cows was determined by macroscopic observation of the ovaries and the uterus. The relative expression of adiponectin, AdipoR1 and AdipoR2 mRNA in theca and cumulus cells were determined by qRT-PCR. Results: Adiponectin and its receptors genes were clearly expressed higher (p<0.05) in theca and cumulus cells of DFs than those of NDFs during the follicular and luteal phases of bovine estrous cycle. Positive correlation (r=0.7; p<0.01) was observed between adiponectin mRNA level in ovarian cells of DFs and follicular fluid estradiol concentration in follicular phase. Adiponectin mRNA abundance in ovarian cells of NDFs showed a significant negative correlation with follicular fluid progesterone concentration in follicular phase (r= -0.7; p<0.01) and luteal phase (r= -0.80, p=0.01). Conclusion: This work has revealed the novel association of adiponectin and its receptors genes with follicular dominance.
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volume 5 issue Supplement Issue
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publication date 2011-09-01
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